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KMID : 0604520220480010047
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Journal of the Society of Cosmetic Scientists of Korea
2022 Volume.48 No. 1 p.47 ~ p.53
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Efficient Extraction Method of Black Tea using Mineral Water and Antioxidant and Anti-inflammatory Activity of Extract
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Kim Eun-Mi
Jung Ji-Yong
Kim Hyung-Min
Hwang Kyeong-Hwan
Park Won-Seok
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Abstract
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In this study, the conditions for efficiently extracting polyphenols contained in black tea were optimized. In order to confirm the polyphenol content according to the degree of fermentation, the polyphenol content in black tea of the three fermentation degrees was compared. As the degree of fermentation increased, the polyphenol content increased, and it was confirmed that the theabrownin content, the largest in size, increased significantly. In addition, it was confirmed that the amount of theabrownin extraction increased by about 150% or more compared to other extraction water when extracting using mineral water, which was hard water. The antioxidant effects of black tea extract and the theabrownin was confirmed using ABTS assay. As a result, both extracts had antioxidant effects, and IC50 values were confirmed to be 10.60 ppm and 13.21 ppm, respectively. And also, the anti-inflammatory effect of the theabrownin was confirmed that the mRNA expression of IL-8 increased by UVB irradiation was inhibited in a concentration dependent manner by the theabrownin.
In addition, when theabrownin was treated with 10 ppm, the mitochondrial function decreased by UVB irradiation was restored to 86 ± 1.9% compared to the control group. Therefore, we concluded that theabrownin could protect mitochondrial damage caused by UVB irradiation.
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KEYWORD
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black tea, mineral water, theabrownin, antioxidant, anti-inflammation
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